According to new legal papers obtained by TMZ, the pair claim they own the rights to hip-hop classic "Looking 4 Da Chewin", and allege G-Eazy's track sampled their 1993 record without permission.
The pair are suing for copyright infringement, and want a slice of the profits from the release, which enjoyed success as the lead single from G-Eazy's fourth studio album, "The Beautiful & Damned".
The Memphis artists' lawyer Alcide Honore told TMZ Juicy J, real name Jordan Michael Houston, was named in the filing because he hails from Memphis, so he should have been aware of the infringement.
He added G-Eazy, real name Gerald Earl Gillum, is included as it's his track, and the other parties could be added to the lawsuit as the case progresses.
It comes after Juicy J and his Three 6 Mafia counterpart DJ Paul were both hit with separate lawsuits earlier this week (begins March 02).