The movie, which banked just over $255 million (£193.5 million) at the worldwide box office, also earned Peele the Best Director title and Best Actress for Lupita Nyong'o.
"Jordan Peele continued to raise the bar in horror specifically and film overall," AAFCA President/Co-Founder Gil Robertson shares in a statement.
"With 'Us', he once again upended the horror genre. His centering of a black nuclear family determined to survive in a complex storyline in a genre where black family units have historically been unseen is extraordinary. He continues to push previously set boundaries with bold storylines that bring a refreshing perspective to cinema overall and the genre specifically."
"The film's $255 million global gross is yet another example that inclusive filmmaking resonates big at the box office and it also resonated critically with our members who awarded the film with our highest honors."
Eddie Murphy was another big winner, taking the Best Actor prize for "Dolemite Is My Name", while his co-star, Da'Vine Joy Randolph, picked up supporting accolades, alongside Jamie Foxx for "Just Mercy".
Along with "Us", "Dolemite Is My Name", "Just Mercy", "The Irishman" and "Clemency" were named the AAFCA's top five best films of the year, while there was also high praise for Impact Award winner "Queen & Slim", "Waves", "Parasite", "Atlantics", "The Farewell" and "Harriet".
Meanwhile, there were double honors for "Waves" as Kelvin Harrison Jr. landed Best Breakout Performance, and Taylor Russell claimed the We See You Award for rising stars.
"Parasite" was declared Best Foreign Film, and its writer/director Bong Joon-ho scored Best Screenplay.
The winners of the AAFCA Awards will be celebrated at a ceremony in Los Angeles on 22 January (20).