The Sao Paulo show served as a tribute to their late brother, Michael Jackson, who is the subject of Dan Reed's damning film into decades of child molestation allegations against the King of Pop.
Performing songs such as "I Want You Back", "ABC", and "Dancing Machine", Tito Jackson announced the show was dedicated to Michael, telling fans, "He is everything and will be represented on the stage."
The family group's South American tour ends in Chile on Saturday, March 23.
The Jacksons are currently suing HBO bosses for airing the "Leaving Neverland" documentary, which features two of Michael's alleged victims, James Safechuck and Wade Robson.
Meanwhile, Paris was hospitalised in Los Angeles on Saturday morning, March 23, after reportedly trying to take her own life.
The 20-year-old and sources close to the family have denied the suicide angle, insisting Jackson, 20, was hospitalised after an accident at her home.